MicroRNA-10a enrichment in factor VIIa-released endothelial extracellular vesicles: potential mechanisms.

ABSTRACT:

Background

Factor VIIa induces the release of extracellular vesicles (EVs) from endothelial cells (EEVs). Factor VIIa-released EEVs are enriched with microRNA-10a (miR10a) and elicit miR10a-dependent cytoprotective responses.

Objectives

To investigate mechanisms by which FVIIa induces miR10a expression in endothelial cells and sorts miR10a into the EVs.

Methods

Activation of Elk-1 and TWIST1 expression was analyzed by immunofluorescence microscopy and immunoblot analysis. Small interfering RNA silencing approach was used to knock down the expression of specific genes in endothelial cells. EVs secreted from endothelial cells or released into circulation in mice were isolated by centrifugation and quantified by nanoparticle tracking analysis. Factor VIIa or EVs were inj

SUBMITTER: Das K 

PROVIDER: S-EPMC10872460 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature