Function and protective capacity of Treponema pallidum subsp. pallidum glycerophosphodiester phosphodiesterase.

ABSTRACT: Infectious syphilis, caused by the spirochete bacterium Treponema pallidum subsp. pallidum, remains a public health concern worldwide. The immune-response evasion mechanisms employed by T. pallidum are poorly understood, and prior attempts to identify immunoprotective antigens for subsequent vaccine design have been unsuccessful. Previous investigations conducted in our laboratory identified the T. pallidum glycerophosphodiester phosphodiesterase as a potential immunoprotective antigen by using a differential immunologic expression library screen. In studies reported here, heterologous expression of the T. pallidum glycerophosphodiester phosphodiesterase in Escherichia coli yielded a full-length, enzymatically active protein. Characterization of the recombinant molecule showed it to be bif

SUBMITTER: Cameron CE 

PROVIDER: S-EPMC108728 | biostudies-literature | 1998 Dec

REPOSITORIES: biostudies-literature