Mitofusin-2 Regulates Platelet Mitochondria and Function.

ABSTRACT:

Background

Single-nucleotide polymorphisms linked with the rs1474868 T allele (MFN2 [mitofusin-2] T/T) in the human mitochondrial fusion protein MFN2 gene are associated with reduced platelet MFN2 RNA expression and platelet counts. This study investigates the impact of MFN2 on megakaryocyte and platelet biology.

Methods

Mice with megakaryocyte/platelet deletion of Mfn2 (Mfn2^-/- [Mfn2 conditional knockout]) were generated using Pf4-Cre crossed with floxed Mfn2 mice. Human megakaryocytes were generated from cord blood and platelets isolated from healthy subjects genotyped for rs1474868. Ex vivo approaches assessed mitochondrial morphology, function, and platelet activation responses. In vivo measurements included end

SUBMITTER: Jacob S 

PROVIDER: S-EPMC10872864 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature