Trans-Endothelial neutrophil migration activates bactericidal function via Piezo1 mechanosensing.

ABSTRACT: The regulation of polymorphonuclear leukocyte (PMN) function by mechanical forces encountered during their migration across restrictive endothelial cell junctions is not well understood. Using genetic, imaging, microfluidic, and in vivo approaches, we demonstrated that the mechanosensor Piezo1 in PMN plasmalemma induced spike-like Ca²⁺ signals during trans-endothelial migration. Mechanosensing increased the bactericidal function of PMN entering tissue. Mice in which Piezo1 in PMNs was genetically deleted were defective in clearing bacteria, and their lungs were predisposed to severe infection. Adoptive transfer of Piezo1-activated PMNs into the lungs of Pseudomonas aeruginosa-infected mice or exposing PMNs to defined mechanical forces in microfluidic systems improved bacterial c

SUBMITTER: Mukhopadhyay A 

PROVIDER: S-EPMC10872880 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature