Effects of Anesthetic Administration on Rat Hypothalamus and Cerebral Cortex Peptidome.

ABSTRACT: Prohormone-derived neuropeptides act as cell-cell signaling molecules to mediate a wide variety of biological processes in the animal brain. Mass spectrometry-based peptidomic experiments are valuable approaches to gain insight into the dynamics of individual peptides under different physiological conditions or experimental treatments. However, the use of anesthetics during animal procedures may confound experimental peptide measurements, especially in the brain, where anesthetics act. Here, we investigated the effects of the commonly used anesthetics isoflurane and sodium pentobarbital on the peptide profile in the rodent hypothalamus and cerebral cortex, as assessed by label-free quantitative peptidomics. Our results showed that neither anesthetic dramatically alters peptide levels, alth

SUBMITTER: Mousavi S 

PROVIDER: S-EPMC10872895 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature