Reductive amination cascades in cell-free and resting whole cell formats for valorization of lignin deconstruction products.

ABSTRACT: The selective introduction of amine groups within deconstruction products of lignin could provide an avenue for valorizing waste biomass while achieving a green synthesis of industrially relevant building blocks from sustainable sources. Here, we built and characterized enzyme cascades that create aldehydes and subsequently primary amines from diverse lignin-derived carboxylic acids using a carboxylic acid reductase (CAR) and an ω-transaminase (TA). Unlike previous studies that have paired CAR and TA enzymes, here we examine multiple homologs of each of these enzymes and a broader set of candidate substrates. In addition, we compare the performance of these systems in cell-free and resting whole-cell biocatalysis formats using the conversion of vanillate to vanillyl amine as model chemistr

SUBMITTER: Nain P 

PROVIDER: S-EPMC10872919 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature