A self-assembling split Nano luciferase-based assay for investigating Pseudomonas syringae effector secretion.

ABSTRACT: Many Gram-negative pathogens employ the type III secretion system (T3SS) to deliver effector proteins into host cells, thereby modulating host cellular processes and suppressing host immunity to facilitate pathogenesis and colonization. In this study, we developed a straightforward, rapid, and quantitative method for detecting T3SS-mediated translocation of Pseudomonas syringae effectors using a self-assembling split Nano luciferase (Nluc)-based reporter system. It was demonstrated that this system can detect effector secretion in vitro with an exceptionally high signal-to-noise ratio and sensitivity, attributed to the strong affinity between the split domains of Nluc and the intense luminescence generated by functional Nluc. During natural infections, effectors fused to a small C-terminal

SUBMITTER: Miao P 

PROVIDER: S-EPMC10873255 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature