Simulated microgravity attenuates myogenesis and contractile function of 3D engineered skeletal muscle tissues.

ABSTRACT: While the effects of microgravity on inducing skeletal muscle atrophy have been extensively studied, the impacts of microgravity on myogenesis and its mechanisms remain unclear. In this study, we developed a microphysiological system of engineered muscle tissue (EMT) fabricated using a collagen / Matrigel composite hydrogel and murine skeletal myoblasts. This 3D EMT model allows non-invasive quantitative assessment of contractile function. After applying a 7-day differentiation protocol to induce myotube formation, the EMTs clearly exhibited sarcomerogenesis, myofilament formation, and synchronous twitch and tetanic contractions with electrical stimuli. Using this 3D EMT system, we investigated the effects of simulated microgravity at 10^-3G on myogenesis and contractile functio

SUBMITTER: Ren Z 

PROVIDER: S-EPMC10873406 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature