RNA m⁶A modification regulates L1 retrotransposons in human spermatogonial stem cell differentiation in vitro and in vivo.

ABSTRACT: The maintenance of genome integrity in the germline is crucial for mammalian development. Long interspersed element type 1 (LINE-1, L1) is a mobile genetic element that makes up about 17% of the human genome and poses a threat to genome integrity. N6-methyl-adenosine (m⁶A) plays an essential role in regulating various biological processes. However, the function of m⁶A modification in L1 retrotransposons and human germline development remains largely unknown. Here we knocked out the m⁶A methyltransferase METTL3 or the m⁶A reader YTHDF2 in human embryonic stem cells (hESCs) and discovered that METTL3 and YTHDF2 are crucial for inducing human spermatogonial stem cells (hSSCs) from hESCs in vitro. The removal of METTL3 or YTHDF2 resulted in increased

SUBMITTER: Li Z 

PROVIDER: S-EPMC10873452 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature