Lskipk Lsatpase double mutants are necessary and sufficient for the compact plant architecture of butterhead lettuce.

ABSTRACT: Lettuce, an important leafy vegetable crop worldwide, has rich variations in plant architecture. Butterhead lettuce, a popular horticultural type, has a unique plant architecture with loose leafy heads. The genetic and molecular mechanisms for such a compact plant architecture remain unclear. In this study we constructed a segregating population through crossing a butterhead cultivar and a stem lettuce cultivar. Genetic analysis identified the LsKIPK gene, which encodes a kinase, as the candidate gene controlling butterhead plant architecture. The Lskipk gene in the butterhead parent had a nonsense mutation, leading to a partial predicted protein. CRISPR/Cas9 and complementation tests verified its functions in plant architecture. We showed that the loss of function of LsKI

SUBMITTER: Xie S 

PROVIDER: S-EPMC10873588 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature