Filament structure and subcellular organization of the bacterial intermediate filament-like protein crescentin.

ABSTRACT: The protein crescentin is required for the crescent shape of the freshwater bacterium Caulobacter crescentus (vibrioides). Crescentin forms a filamentous structure on the inner, concave side of the curved cells. It shares features with eukaryotic intermediate filament (IF) proteins, including the formation of static filaments based on long and parallel coiled coils, the protein's length, structural roles in cell and organelle shape determination and the presence of a coiled coil discontinuity called the "stutter." Here, we have used electron cryomicroscopy (cryo-EM) to determine the structure of the full-length protein and its filament, exploiting a crescentin-specific nanobody. The filament is formed by two strands, related by twofold symmetry, that each consist of two dimer

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10873595 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature