An updated and extended version of the Melastomataceae probe set for target capture.

ABSTRACT:

Premise

A probe set was previously designed to target 384 nuclear loci in the Melastomataceae family; however, when trying to use it, we encountered several practical and conceptual problems, such as the presence of sequences in reverse complement, intronic regions with stop codons, and other issues. This raised concerns regarding the use of this probe set for sequence recovery in Melastomataceae.

Methods

In order to correct these issues, we cleaned the Melastomataceae probe set, extended it with additional sequences, and compared its performance with the original version.

Results

The final probe set targets 396 putative nuclear loci represented by 6009 template sequences. The probe set has been made available, along with details on the cleaning process, for reproduci

SUBMITTER: Dagallier LMJ 

PROVIDER: S-EPMC10873813 | biostudies-literature | 2024 Jan-Feb

REPOSITORIES: biostudies-literature