Modularly assembled multiplex prime editors for simultaneous editing of agronomically important genes in rice.

ABSTRACT: Prime editing (PE) technology enables precise alterations in the genetic code of a genome of interest. PE offers great potential for identifying major agronomically important genes in plants and editing them into superior variants, ideally targeting multiple loci simultaneously to realize the collective effects of the edits. Here, we report the development of a modular assembly-based multiplex PE system in rice and demonstrate its efficacy in editing up to four genes in a single transformation experiment. The duplex PE (DPE) system achieved a co-editing efficiency of 46.1% in the T₀ generation, converting TFIIAγ5 to xa5 and xa23 to Xa23^SW11. The resulting double-mutant lines exhibited robust broad-spectrum resistance against multiple Xanthomonas oryzae pathovar oryzae

SUBMITTER: Gupta A 

PROVIDER: S-EPMC10873889 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature