Injection-based hairy root induction and plant regeneration techniques in Brassicaceae.

ABSTRACT:

Background

Hairy roots constitute a valuable tissue culture system for species that are difficult to propagate through conventional seed-based methods. Moreover, the generation of transgenic plants derived from hairy roots can be facilitated by employing carefully designed hormone-containing media.

Results

We initiated hairy root formation in the rare crucifer species Asperuginoides axillaris via an injection-based protocol using the Agrobacterium strain C58C1 harboring a hairy root-inducing (Ri) plasmid and successfully regenerated plants from established hairy root lines. Our study confirms the genetic stability of both hairy roots and their derived regenerants and highlights their utility as a permanent source of mitotic chromosomes for cytogenetic investigations. Additio

SUBMITTER: Jedlickova V 

PROVIDER: S-EPMC10874044 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature