Phytol from Scoparia dulcis prevents NF-κB-mediated inflammatory responses during macrophage polarization.

ABSTRACT: Macrophages are primary immune cells that mediate a wide range of inflammatory diseases through their polarization potential. In this study, phytol isolated from Scoparia dulcis has been explored against 7-ketocholesterol and bacterial lipopolysaccharide-induced macrophage polarization in IC-21 cells. Isolated phytol has been characterized using GC-MS, TLC, HPTLC, FTIR, ¹H-NMR, and HPLC analyses. The immunomodulatory effects of viable concentrations of phytol were tested on oxidative stress, arginase activity, nuclear and mitochondrial membrane potentials in IC-21 cells in addition to the modulation of calcium and lipids. Further, gene and protein expression of atherogenic markers were studied. Results showed that the isolated phytol at a viable concentration of 400 µg/ml

SUBMITTER: Duraisamy P 

PROVIDER: S-EPMC10874368 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature