Perturbation of Wnt/β-catenin signaling and sexual dimorphism in non-alcoholic fatty liver disease.

ABSTRACT:

Aims

The prevalence of non-alcoholic fatty liver disease (NAFLD) and its progression to non-alcoholic steatohepatitis (NASH) is higher in postmenopausal women than men. The aim of this study was to determine the molecular mechanisms underlying this sexual dimorphism in NAFLD.

Methods

A total of 24 frozen liver samples of both sexes (normal and NAFLD/NASH) were used in this study. Total RNAseq was first used to identify differentially expressed genes (DEGs) between samples. Enrichment analysis of Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome were used to analyze biological pathways. RT² profiler polymerase chain reaction (PCR) arrays were used to identify genes associated with the biological pathways. Immunoblotting was used to va

SUBMITTER: Yeh MM 

PROVIDER: S-EPMC10874498 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature