Scalable Fabrication and Use of 3D Structured Microparticles Spatially Functionalized with Biomolecules.

ABSTRACT: Microparticles with defined shapes and spatial chemical modification can interface with cells and tissues at the cellular scale. However, conventional methods to fabricate shaped microparticles have trade-offs between the throughput of manufacture and the precision of particle shape and chemical functionalization. Here, we achieved scalable production of hydrogel microparticles at rates of greater than 40 million/hour with localized surface chemistry using a parallelized step emulsification device and temperature-induced phase-separation. The approach harnesses a polymerizable polyethylene glycol (PEG) and gelatin aqueous two-phase system (ATPS) which conditionally phase separates within microfluidically generated droplets. Following droplet formation, phase separation is induced and phase

SUBMITTER: Lee S 

PROVIDER: S-EPMC10874522 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature