Biosynthesis of melatonin from L-tryptophan by an engineered microbial cell factory.

ABSTRACT:

Background

The demand for melatonin is increasing due to its health-promoting bioactivities such as antioxidant and sleep benefits. Although melatonin is present in various organisms, its low content and high extraction cost make it unsustainable. Biosynthesis is a promising alternative method for melatonin production. However, the ectopic production of melatonin in microorganisms is very difficult due to the low or insoluble expression of melatonin synthesis genes. Hence, we aim to explore the biosynthesis of melatonin using Escherichia coli as a cell factory and ways to simultaneously coordinated express genes from different melatonin synthesis pathways.

Results

In this study, the mXcP4H gene from Xanthomonas campestris, as well as the HsAADC, HsAANAT and HIOMT genes from

SUBMITTER: Wang L 

PROVIDER: S-EPMC10874579 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature