Ratiometric Detection of Zn²⁺ Using DNAzyme-Based Bioluminescence Resonance Energy Transfer Sensors.

ABSTRACT: While fluorescent sensors have been developed for monitoring metal ions in health and diseases, they are limited by the requirement of an excitation light source that can lead to photobleaching and a high autofluorescence background. To address these issues, bioluminescence resonance energy transfer (BRET)-based protein or small molecule sensors have been developed; however, most of them are not highly selective nor generalizable to different metal ions. Taking advantage of the high selectivity and generalizability of DNAzymes, we report herein DNAzyme-based ratiometric sensors for Zn²⁺ based on BRET. The 8-17 DNAzyme was labeled with luciferase and Cy3. The proximity between luciferase and Cy3 permiQed BRET when coelenterazine, the substrate for luciferase, was introduced. Addi

SUBMITTER: Wu Y 

PROVIDER: S-EPMC10874629 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature