Development and validation of a multiplexed LC-MS/MS ketone body assay for clinical diagnostics.

ABSTRACT:

Objectives

Ketone bodies (KBs) serve as important energy sources that spare glucose, providing the primary energy for cardiac muscle, skeletal muscle during aerobic exercise, and the brain during periods of catabolism. The levels and relationships between the KBs are critical indicators of metabolic health and disease. However, challenges in separating isomeric KBs and concerns about sample stability have previously limited their clinical measurement.

Methods

A novel 6.5-minute liquid chromatography-mass spectrometry-based assay was developed, enabling the precise measurement of alpha-, beta- and gamma-hydroxybutyrate, beta-hydroxyisobutyrate, and acetoacetate. This method was fully validated for human serum and plasma samples by investigating extraction efficiency, matrix e

SUBMITTER: Kemperman RHJ 

PROVIDER: S-EPMC10874984 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature