The sequence of events of enteropathogenic E. coli's type III secretion system translocon assembly.

ABSTRACT: Many bacterial pathogens employ the type III secretion system (T3SS), a specialized complex that transports effector proteins that manipulate various cellular processes. The T3SS forms a translocon pore within the host-cell membrane consisting of two secreted proteins that transition from a soluble state into a transmembrane complex. Still, the exact sequence of events leading to the formation of a membranous functional pore remains uncertain. Here, we utilized the translocon proteins of enteropathogenic E. coli (EPEC) to investigate the sequence of those steps leading to translocon assembly, including self-oligomerization, hetero-oligomerization, interprotein interaction, and membrane insertion. We found that in EPEC, EspD (SctE) plays a dominant role in pore formation as it assemb

SUBMITTER: Gershberg J 

PROVIDER: S-EPMC10875159 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature