Design and validation of a custom-made system to measure transepithelial electrical impedance in human corneas preserved in active storage machine.

ABSTRACT: Corneal epithelial barrier represents one of the major limitations to ocular drug delivery and can be explored non-invasively through the evaluation of its electrical properties. Human corneas stored in active storage machine (ASM) could represent an interesting physiological model to explore transcorneal drug penetration. We designed a new system adapted to human corneas preserved in ASM to explore corneal epithelial barrier function ex-vivo. A bipolar set-up including Ag/AgCl electrodes adaptors to fit the corneal ASM and a dedicated software was designed and tested on freshly excised porcine corneas (n = 59) and human corneas stored 14 days in ASM (n = 6). Porcine corneas presented significant and proportional decrease in corneal impedance in response to increasing-size ep

SUBMITTER: Mentek M 

PROVIDER: S-EPMC10875219 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature