Large-scale loss-of-function perturbations reveal a comprehensive epigenetic regulatory network in breast cancer.

ABSTRACT:

Objective

Epigenetic abnormalities have a critical role in breast cancer by regulating gene expression; however, the intricate interrelationships and key roles of approximately 400 epigenetic regulators in breast cancer remain elusive. It is important to decipher the comprehensive epigenetic regulatory network in breast cancer cells to identify master epigenetic regulators and potential therapeutic targets.

Methods

We employed high-throughput sequencing-based high-throughput screening (HTS²) to effectively detect changes in the expression of 2,986 genes following the knockdown of 400 epigenetic regulators. Then, bioinformatics analysis tools were used for the resulting gene expression signatures to investigate the epigenetic regulations in breast cancer.

Result

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10875281 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature