Methane-oxidizing bacteria in a California upland grassland soil: diversity and response to simulated global change.

ABSTRACT: We investigated the diversity of methane-oxidizing bacteria (i.e., methanotrophs) in an annual upland grassland in northern California, using comparative sequence analysis of the pmoA gene. In addition to identifying type II methanotrophs commonly found in soils, we discovered three novel pmoA lineages for which no cultivated members have been previously reported. These novel pmoA clades clustered together either with clone sequences related to "RA 14" or "WB5FH-A," which both represent clusters of environmentally retrieved sequences of putative atmospheric methane oxidizers. Conservation of amino acid residues and rates of nonsynonymous versus synonymous nucleotide substitution in these novel lineages suggests that the pmoA genes in these clades code for functionally active methane monoox

SUBMITTER: Horz HP 

PROVIDER: S-EPMC1087552 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature