DNA microarray analysis of nitrogen fixation and Fe(III) reduction in Geobacter sulfurreducens.

ABSTRACT: A DNA microarray representing the genome of Geobacter sulfurreducens was constructed for use in global gene expression profiling of cells under steady-state conditions with acetate as the electron donor and Fe(III) or fumarate as the electron acceptor. Reproducible differences in transcript levels were also observed in comparisons between cells grown with ammonia and those fixing atmospheric nitrogen. There was a high correlation between changes in transcript levels determined with microarray analyses and an evaluation of a subset of the genome with quantitative PCR. As expected, cells required to fix nitrogen had higher levels of transcripts of genes associated with nitrogen fixation, further demonstrating that the microarray approach could reliably detect important physiological changes.

SUBMITTER: Methe BA 

PROVIDER: S-EPMC1087574 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature