Metabolic engineering of Synechocystis sp. PCC 6803 for the improved production of phenylpropanoids.

ABSTRACT:

Background

Phenylpropanoids are a large group of plant secondary metabolites with various biological functions, derived from aromatic amino acids. Cyanobacteria are promising host organisms for sustainable production of plant phenylpropanoids. We have previously engineered Synechocystis sp. PCC 6803 to produce trans-cinnamic acid (tCA) and p-coumaric acid (pCou), the first intermediates of phenylpropanoid pathway, by overexpression of phenylalanine- and tyrosine ammonia lyases. In this study, we aimed to enhance the production of the target compounds tCA and pCou in Synechocystis.

Results

We eliminated the 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity, which is a competing pathway consuming tyrosine and, possibly, phenylalanine for tocopherol synthesis. Moreover, sever

SUBMITTER: Kukil K 

PROVIDER: S-EPMC10875765 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature