A resource database for protein kinase substrate sequence-preference motifs based on large-scale mass spectrometry data.

ABSTRACT:

Background

Protein phosphorylation is one of the most prevalent posttranslational modifications involved in molecular control of cellular processes, and is mediated by over 520 protein kinases in humans and other mammals. Identification of the protein kinases responsible for phosphorylation events is key to understanding signaling pathways. Unbiased phosphoproteomics experiments have generated a wealth of data that can be used to identify protein kinase targets and their preferred substrate sequences.

Methods

This study utilized prior data from mass spectrometry-based studies identifying sites of protein phosphorylation after in vitro incubation of protein mixtures with recombinant protein kinases. PTM-Logo software was used with these data to generate position-dependent Sha

SUBMITTER: Poll BG 

PROVIDER: S-EPMC10875805 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature