Effects of continuous and released compressive force on osteoclastogenesis invitro.

ABSTRACT:

Objective

Compressive force has been found to be catabolic to alveolar bone during orthodontic tooth movement. This study quantified the fusion of mononuclear RAW 264.7 cells (a murine osteoclastic-like cell line) into multinucleated osteoclasts under a hydrostatic pressure-generated mechanical compression-the new model of various magnitudes and durations.

Methods

RAW 264.7 cells were subjected to 0.3, 0.6 or 0.9 g/cm² of compressive force by an acrylic cylinder custom-made by laser cutting or no compressive force for 4 days during osteoclastogenic induction. TRAP-positive multinucleated cells were quantified. For the release from force experiment, osteoclastogenesis was induced by 0.6 g/cm² mechanical stimuli for 0, 1, 2, 3 or 4 days. Cell viability, T

SUBMITTER: Changkhaokham B 

PROVIDER: S-EPMC10876609 | biostudies-literature | 2024 Mar-Apr

REPOSITORIES: biostudies-literature