Glutamate is effective in decreasing opacity formed in galactose-induced cataract model.

ABSTRACT: Although cataract is the leading cause of blindness worldwide, the detailed pathogenesis of cataract remains unclear, and clinically useful drug treatments are still lacking. In this study, we examined the effects of glutamate using an ex vivo model in which rat lens is cultured in a galactose-containing medium to induce opacity formation. After inducing lens opacity formation in galactose medium, glutamate was added, and the opacity decreased when the culture was continued. Next, microarray analysis was performed using samples in which the opacity was reduced by glutamate, and genes whose expression increased with galactose culture and decreased with the addition of glutamate were extracted. Subsequently, STRING analysis was performed on a group of genes that showed variation as a result

SUBMITTER: Takashima M 

PROVIDER: S-EPMC10876653 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature