Characterization of non-invasive oropharyngeal samples and nucleic acid isolation for molecular diagnostics.

ABSTRACT: Molecular diagnostics is an increasingly important clinical tool, especially in routine sampling. We evaluated two non-invasive methods (oral swabs and mouthwashes) for sampling nucleic acids from the oral/pharyngeal area. We created a workflow from sample collection (n = 59) to RT-qPCR based analysis. The samples were further characterized in terms of their cellular composition as well as the purity, degradation and microbial content of the derived DNA/RNA. We determined the optimal housekeeping genes applicable for these types of samples. The cellular composition indicated that mouthwashes contained more immune cells and bacteria. Even though the protocol was not specifically optimized to extract bacterial RNA it was possible to derive microbial RNA, from both sampling methods. Optimizin

SUBMITTER: Hose L 

PROVIDER: S-EPMC10876689 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature