Activation of renal epithelial Na⁺ channels (ENaC) in infants with congenital heart disease.

ABSTRACT:

Introduction

This study was designed to measure the concentration and activity of urinary proteases that activate renal epithelial sodium channel (ENaC) mediated Na⁺ transport in infants with congenital heart disease, a potential mechanism for fluid retention.

Methods

Urine samples from infants undergoing cardiac surgery were collected at three time points: T1) pre-operatively, T2) 6-8 h after surgery, and T3) 24 h after diuretics. Urine was collected from five heathy infant controls. The urine was tested for four proteases and whole-cell patch-clamp testing was conducted in renal collecting duct M-1 cells to test whether patient urine increased Na⁺ currents consistent with ENaC activation.

Results

Heavy chain of plasminogen, furin, and prostasin

SUBMITTER: Ortmann LA 

PROVIDER: S-EPMC10876900 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature