In vitro SELEX and application of an African swine fever virus (ASFV) p30 protein specific aptamer.

ABSTRACT: The African swine fever virus (ASFV) has caused severe economic losses in the pig industry. To monitor ASFV spread, the p30 protein has been identified as an ideal infection marker due to its early and long-term expression during the ASFV infection period. Timely monitoring of ASFV p30 enables the detection of ASFV infection and assessment of disease progression. Aptamers are an outstanding substitute for antibodies to develop an efficient tool for ASFV p30 protein detection. In this study, a series of aptamer candidates were screened by in vitro magnetic bead-based systematic evolution of ligands by exponential enrichment (MB-SELEX). An aptamer (Atc-20) finally showed high specificity and affinity (K_d = 140 ± 10 pM) against ASFV p30 protein after truncation and affinity assessm

SUBMITTER: Hu C 

PROVIDER: S-EPMC10876938 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature