Multiplexed CRISPR-Cas9 protocol for large transgene integration into the Schistosoma mansoni genome.
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ABSTRACT:
SUBMITTER: Ittiprasert W
PROVIDER: S-EPMC10876972 | biostudies-literature | 2024 Feb
REPOSITORIES: biostudies-literature
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Multiplexed CRISPR-Cas9 protocol for large transgene integration into the Schistosoma mansoni genome.
Ittiprasert Wannaporn W Moescheid Max M MM Mann Victoria H VH Brindley Paul J PJ
STAR protocols 20240213 1
Precise, on-target CRISPR-Cas9 genome editing has been shown in Schistosoma mansoni, involving both non-homology end joining and homology-directed repair pathways. Here, we present a multiplexed CRISPR-Cas9 protocol for large transgene integration into the S. mansoni genome. We describe steps for deploying multiplexed ribonucleoprotein complexes (RNPs) and donor DNA preparation. We then detail procedures for introducing RNPs into schistosome eggs by square-wave electroporation in the presence of ...[more]
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