Detection of Candidatus Liberibacter asiaticus and five viruses in individual Asian citrus psyllid in China.

ABSTRACT:

Introduction

Asian citrus psyllid (ACP, Diaphorina citri) is an important transmission vector of "Candidatus Liberibacter asiaticus" (CLas), the causal agent of Huanglongbing (HLB), the most destructive citrus disease in the world. As there are currently no HLB-resistant rootstocks or varieties, the control of ACP is an important way to prevent HLB. Some viruses of insect vectors can be used as genetically engineered materials to control insect vectors.

Methods

To gain knowledge on viruses in ACP in China, the prevalence of five RNA and DNA viruses was successfully determined by optimizing reverse transcription polymerase chain reaction (RT-PCR) in individual adult ACPs. The five ACP-associated viruses were identified as follows: diaphorina citri bunyavi

SUBMITTER: Liu L 

PROVIDER: S-EPMC10877018 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature