Promoter engineering enables precise metabolic regulation towards efficient β-elemene production in Ogataea polymorpha.

ABSTRACT: Precisely controlling gene expression is beneficial for optimizing biosynthetic pathways for improving the production. However, promoters in nonconventional yeasts such as Ogataea polymorpha are always limited, which results in incompatible gene modulation. Here, we expanded the promoter library in O. polymorpha based on transcriptional data, among which 13 constitutive promoters had the strengths ranging from 0-55% of P_GAP, the commonly used strong constitutive promoter, and 2 were growth phase-dependent promoters. Subsequently, 2 hybrid growth phase-dependent promoters were constructed and characterized, which had 2-fold higher activities. Finally, promoter engineering was applied to precisely regulate cellular metabolism for efficient production of β-elem

SUBMITTER: Ye M 

PROVIDER: S-EPMC10877135 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature