"In-situ" formation of elastin-like recombinamer hydrogels with tunable viscoelasticity through efficient one-pot process.

ABSTRACT: Despite the remarkable progress in the generation of recombinant elastin-like (ELR) hydrogels, further improvements are still required to enhance and control their viscoelasticity, as well as limit the use of expensive chemical reagents, time-consuming processes and several purification steps. To alleviate this issue, the reactivity of carboxylic groups from glutamic (E) acid distributed along the hydrophilic block of an amphiphilic ELR (coded as E50I60) with amine groups has been studied through a one-pot amidation reaction in aqueous solutions, for the first time. By means of this approach, immediate conjugation of E50I60 with molecules containing amine groups has been performed with a high yield, as demonstrated by the ¹H NMR and MALDI-TOF spectroscopies. This has resulted in

SUBMITTER: Misbah MH 

PROVIDER: S-EPMC10877175 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature