Naturally occurring PA^E206K point mutation in 2009 H1N1 pandemic influenza viruses impairs viral replication at high temperatures.

ABSTRACT: The emergence of influenza virus A pandemic H1N1 in April 2009 marked the first pandemic of the 21st century. In this study, we observed significant differences in the polymerase activities of two clinical 2009 H1N1 influenza A virus isolates from Chinese and Japanese patients. Sequence comparison of the three main protein subunits (PB2, PB1, and PA) of the viral RNA-dependent RNA polymerase complex and subsequent mutational analysis revealed that a single amino acid substitution (E206K) was responsible for the observed impaired replication phenotype. Further in vitro experiments showed that presence of PA^E206K decreased the replication of influenza A/WSN/33 virus in mammalian cells and a reduction in the virus's pathogenicity in vivo. Mechanistic studies revealed that PA^E2

SUBMITTER: Cao M 

PROVIDER: S-EPMC10877435 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature