POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic Biology.

ABSTRACT: The fission yeast Schizosaccharomyces pombe is a popular model organism in molecular biology and cell physiology. With its ease of genetic manipulation and growth, supported by in-depth functional annotations in the PomBase database and genome-wide metabolic models,S. pombe is an attractive option for synthetic biology applications. However,S. pombe currently lacks modular tools for generating genetic circuits with more than 1 transcriptional unit. We developed a toolkit to address this gap. Adapted from the MoClo-YTK plasmid kit for Saccharomyces cerevisiae and using the same modular cloning grammar, our POMBOX toolkit is designed to facilitate fast, efficient, and modular construction of genetic circuits inS. pombe. It allows for interoperability when w

SUBMITTER: Hebra T 

PROVIDER: S-EPMC10877588 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature