Effect of TraN key residues involved in DNA binding on pIP501 transfer rates in <i>Enterococcus faecalis</i>.

ABSTRACT: Conjugation is a major mechanism that facilitates the exchange of antibiotic resistance genes among bacteria. The broad-host-range Inc18 plasmid pIP501 harbors 15 genes that encode for a type IV secretion system (T4SS). It is a membrane-spanning multiprotein complex formed between conjugating donor and recipient cells. The penultimate gene of the pIP501 operon encodes for the cytosolic monomeric protein TraN. This acts as a transcriptional regulator by binding upstream of the operon promotor, partially overlapping with the origin of transfer. Additionally, TraN regulates traN and traO expression by binding upstream of the P_traNO promoter. This study investigates the impact of nine TraN amino acids involved in binding to pIP501 DNA through site-directed mutag

SUBMITTER: Michaelis C 

PROVIDER: S-EPMC10877727 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature