Diagnostic application of padlock probes--multiplex detection of plant pathogens using universal microarrays.

ABSTRACT: Padlock probes (PLPs) are long oligonucleotides, whose ends are complementary to adjacent target sequences. Upon hybridization to the target, the two ends are brought into contact, allowing PLP circularization by ligation. PLPs provide extremely specific target recognition, which is followed by universal amplification and microarray detection. Since target recognition is separated from downstream processing, PLPs enable the development of flexible and extendable diagnostic systems, targeting diverse organisms. To adapt padlock technology for diagnostic purposes, we optimized PLP design to ensure high specificity and eliminating ligation on non-target sequences under real-world assay conditions. We designed and tested 11 PLPs to target various plant pathogens at the genus, species and subsp

SUBMITTER: Szemes M 

PROVIDER: S-EPMC1087788 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature