Genome sequencing unveils bla_KPC-2-harboring plasmids as drivers of enhanced resistance and virulence in nosocomial Klebsiella pneumoniae.

ABSTRACT: The threat posed by Klebsiella pneumoniae in healthcare settings has worsened due to the evolutionary advantages conferred by bla_KPC-2-harboring plasmids (pKPC-2). However, the specific evolutionary pathway of nosocomial K. pneumoniae carrying pKPC-2 and its transmission between patients and healthcare environments are not yet well understood. Between 1 August and 31 December 2019, 237 ST11 KPC-2-producing-carbapenem-resistant K. pneumoniae (CRKP) (KPC-2-CRKP) were collected from patient or ward environments in an intensive care unit and subjected to Illumina sequencing, of which 32 strains were additionally selected for Nanopore sequencing to obtain complete plasmid sequences. Bioinformatics analysis, conjugation experiments, antimicrobial susceptibi

SUBMITTER: Han X 

PROVIDER: S-EPMC10878039 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature