Single-cell analysis of MYD88^L265P and MYD88^WT Waldenstrom macroglobulinemia patients.

ABSTRACT: Waldenström macroglobulinemia (WM) is characterized by the expansion of clonal lymphoplasmacytic cells; the MYD88L265P somatic mutation is found in >90% of patients, but malignant B cells may still display intra-clonal heterogeneity. To assess clonal heterogeneity in WM, we generated and performed single-cell RNA sequencing of CD19⁺ sorted cells from five patients with MYD88 ^L265P and two patients with MYD88 ^WT genotype as well as two healthy donors. We identified distinct transcriptional patterns in the clonal subpopulations not only between the two genetically distinct WM subgroups but also among MYD88 ^L265P patients, which affected the B cell composition in the different subgroups. Comparison of clonal

SUBMITTER: Bagratuni T 

PROVIDER: S-EPMC10878187 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature