Gut Microbiota Richness and Diversity Track With T Cell Aging in Healthy Adults.

ABSTRACT:

Background

This study examined how gut microbiota diversity and richness relate to T cell aging among 96 healthy adults of all ages. It also explored whether these links differed throughout the lifespan.

Methods

Peripheral blood was obtained from 96 study participants (N = 96, aged 21-72) to assess mRNA markers of T cell aging (p16ink4a, p14ARF, B3gat1, Klrg1) and DNA methylation. T cell aging mRNA markers were combined into an aging index, and the Horvath epigenetic clock algorithm was used to calculate epigenetic age based on DNA methylation status of over 500 loci. Participants also collected a stool sample from which the V4 region of the 16S rRNA gene was sequenced to derive the Shannon and Simpson diversity indices, and the total count of observed operational taxonomic

SUBMITTER: Madison AA 

PROVIDER: S-EPMC10878250 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature