PUdOs: Concise Plasmids for Bacterial and Mammalian Cells.

ABSTRACT: The plasmids from the Université d'Ottawa (pUdOs) are 28 small plasmids each comprising one of four origins of replication and one of seven selection markers, which together afford flexible use in Escherichia coli and several related gram-negative bacteria. The promoterless multicloning site is insulated from upstream spurious promoters by strong transcription terminators and contains type IIP or IIS restriction sites for conventional or Golden Gate cloning. pUdOs can be converted into efficient expression vectors through the insertion of a promoter at the user's discretion. For example, we demonstrate the utility of pUdOs as the backbone for an improved version of a Type III Secretion System reporter in Shigella. In addition, we derive a series of pUdO-based mammalian expres

SUBMITTER: Manigat FO 

PROVIDER: S-EPMC10878396 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature