Sperm Toolbox-A selection of small molecules to study human spermatozoa.

ABSTRACT: Male contraceptive options and infertility treatments are limited, and almost all innovation has been limited to updates to medically assisted reproduction protocols and methods. To accelerate the development of drugs that can either improve or inhibit fertility, we established a small molecule library as a toolbox for assay development and screening campaigns using human spermatozoa. We have profiled all compounds in the Sperm Toolbox in several automated high-throughput assays that measure stimulation or inhibition of sperm motility or the acrosome reaction. We have assayed motility under non-capacitating and capacitating conditions to distinguish between pathways operating under these different physiological states. We also assayed cell viability to ensure any effects on sperm function

SUBMITTER: Gruber FS 

PROVIDER: S-EPMC10878532 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature