Imaging plant cell death: GFP-Nit1 aggregation marks an early step of wound and herbicide induced cell death.

ABSTRACT:

Background

A great deal is known about the morphological endpoints of plant cell death, but relatively little is known about its sequence of events and/or its execution at the biochemical level. Live cell imaging using GFP-tagged markers is a powerful way to provide dynamic portraits of a cellular process that can in turn provide a descriptive foundation valuable for future biochemical and genetic investigations.

Results

While characterizing a collection of random GFP-protein fusion markers we discovered that mechanical wounding induces rapid aggregation of a GFP-Nitrilase 1 fusion protein in Arabidopsis cells directly abutting wound sites. Time-lapse imaging of this response shows that the aggregation occurs in cells that subsequently die 30-60 minutes post-wounding, indica

SUBMITTER: Cutler SR 

PROVIDER: S-EPMC1087855 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature