Quantifying the minimum localization uncertainty of image scanning localization microscopy.

ABSTRACT: Modulation enhanced single-molecule localization microscopy (meSMLM), where emitters are sparsely activated with sequentially applied patterned illumination, increases the localization precision over single-molecule localization microscopy (SMLM). The precision improvement of modulation enhanced SMLM is derived from retrieving the position of an emitter relative to individual illumination patterns, which adds to existing point spread function information from SMLM. Here, we introduce SpinFlux: modulation enhanced localization for spinning disk confocal microscopy. SpinFlux uses a spinning disk with pinholes in its illumination and emission paths, to sequentially illuminate regions in the sample during each measurement. The resulting intensity-modulated emission signal is analyzed for each

SUBMITTER: Kalisvaart D 

PROVIDER: S-EPMC10878846 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature