[Bioinformatics analysis and validation of the interaction between PML protein and TAB1 protein].

ABSTRACT:

Objective

To analyze the interaction between PML protein and TAB1 protein using bioinformatic approaches and experimentally verify the results.

Methods

Using Rosetta software, a 3D model of TAB1 protein was constructed through a comparative modeling approach; the secondary structure of PML protein was retrieved in the PDB database and its crystal structure and 3D structure were resolved. Zdock 3.0.2 software was used to perform protein-protein docking of PML and TAB1, and the best conformation was extracted for molecular structure analysis of the docking model. The interaction between the two proteins was detected using immunoprecipitation in α-MMC-treated M1 inflammatory macrophages.

Results

When 6IMQ of PML was used as the docking site, PML protein formed 3 salt bri

SUBMITTER: Cheng J 

PROVIDER: S-EPMC10878890 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature