Spatial lipidomics of coronary atherosclerotic plaque development in a familial hypercholesterolemia swine model.

ABSTRACT: Coronary atherosclerosis is caused by plaque build-up, with lipids playing a pivotal role in its progression. However, lipid composition and distribution within coronary atherosclerosis remain unknown. This study aims to characterize lipids and investigate differences in lipid composition across disease stages to aid in the understanding of disease progression. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was used to visualize lipid distributions in coronary artery sections (n = 17) from hypercholesterolemic swine. We performed histology on consecutive sections to classify the artery segments and to investigate colocalization between lipids and histological regions of interest in advanced plaque, including necrotic core and inflammatory cells. Segments

SUBMITTER: Slijkhuis N 

PROVIDER: S-EPMC10879031 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature