Unsupervised classification of brain-wide axons reveals the presubiculum neuronal projection blueprint.

ABSTRACT: We present a quantitative strategy to identify all projection neuron types from a given region with statistically different patterns of anatomical targeting. We first validate the technique with mouse primary motor cortex layer 6 data, yielding two clusters consistent with cortico-thalamic and intra-telencephalic neurons. We next analyze the presubiculum, a less-explored region, identifying five classes of projecting neurons with unique patterns of divergence, convergence, and specificity. We report several findings: individual classes target multiple subregions along defined functions; all hypothalamic regions are exclusively targeted by the same class also invading midbrain and agranular retrosplenial cortex; Cornu Ammonis receives input from a single class of presubicular axons also pro

SUBMITTER: Wheeler DW 

PROVIDER: S-EPMC10879163 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature